August 11, 2020 in Uncategorized

Direct comparison of five different 3D extracellular matrix model systems for characterization of cancer cell migration

Yoshinari Shinsato, Andrew D. Doyle, Weimin Li, Kenneth M. Yamada Abstract Background Three‐dimensional (3D) in vitro model systems can bridge the gap between regular two‐dimensional cell culture and whole‐animal studies.…
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August 11, 2020 in Uncategorized

Distinct phenotypes of cancer cells on tissue matrix gel

Kelsey F. Ruud, William C. Hiscox, Ilhan Yu, Roland K. Chen & Weimin Li Abstract Breast cancer cells invading the connective tissues outside the mammary lobule or duct immerse in…
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July 30, 2020 in Publications

Simplifying the extracellular matrix for 3‐D cell culture and tissue engineering: A pragmatic approach

Glenn D. Prestwich Abstract The common technique of growing cells on tissue culture plastic (TCP) is gradually being supplanted by methods for culturing cells in two‐dimensions (2‐D) on matrices with…
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July 29, 2020 in Publications

Engineering a clinically-useful matrix for cell therapy

Glenn D. Prestwich Abstract The design criteria for matrices for encapsulation of cells for cell therapy include chemical, biological, engineering, marketing, regulatory, and financial constraints. What is required is a…
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July 29, 2020 in Publications

Dynamically Crosslinked Gold Nanoparticle – Hyaluronan Hydrogels

Aleksander Skardal, Jianxing Zhang, Lindsi McCoard, Siam Oottamasathien, Glenn D. Prestwich Abstract Bioprinting consists of automated deposition of cells and biomaterials to mimic the architecture of a tissue. Gold nanoparticles act…
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July 29, 2020 in Publications

The generation of 3-D tissue models based on hyaluronan hydrogel-coated microcarriers within a rotating wall vessel bioreactor

Aleksander Skardal, Shameema F. Sarker, Aurélie Crabbé, Cheryl A. Nickerson, Glenn D. Prestwich Abstract With the increasing necessity for functional tissue- and organ equivalents in the clinic, the optimization of…
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July 29, 2020 in Publications

Hyaluronic acid-based clinical biomaterials derived for cell and molecule delivery in regenerative medicine

Glenn D.Prestwich Abstract The development of injectable and biocompatible vehicles for delivery, retention, growth, and differentiation of stem cells is of paramount importance for regenerative medicine. For cell therapy and…
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July 29, 2020 in Publications

Hyaluronic Acid Hydrogels for Biomedical Applications

Jason A. Burdick & Glenn D. Prestwich Abstract Hyaluronic acid (HA), an immunoneutral polysaccharide that is ubiquitous in the human body, is crucial for many cellular and tissue functions and…
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July 29, 2020 in Publications

A 3-D organoid kidney culture model engineered for high-throughput nephrotoxicity assays

Anna I. Astashkina, Brenda K. Mann, Glenn D. Prestwich, David W. Grainger Abstract Cell–cell and cell-matrix interactions control cell phenotypes and functions in vivo. Maintaining these interactions in vitro is essential to both produce…
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July 29, 2020 in Publications

Comparing predictive drug nephrotoxicity biomarkers in kidney 3-D primary organoid culture and immortalized cell lines

Anna I.Astashkina, Brenda K.Mann, Glenn D.Prestwich, David W.Grainger Abstract The cellular microenvironment is recognized to play a key role in stabilizing cell differentiation states and phenotypes in culture. This study…
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July 29, 2020 in Publications

The translational imperative: Making cell therapy simple and effective

Glenn D. Prestwich, Isaac E.Erickson, Thomas I. Zarembinski, Michael West, William P.Tew Abstract The current practice of cell therapy, in which multipotent or terminally differentiated cells are injected into tissues…
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July 29, 2020 in Publications

Why regenerative medicine needs an extracellular matrix

Glenn D Prestwich & Kevin E Healy Abstract Regenerative medicine is now coming of age. Many attempts at cell therapy have failed to show significant efficacy, and the umbrella term…
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July 29, 2020 in Publications

Recent advances in hyaluronic acid hydrogels for biomedical applications

Christopher B Highley, Glenn D Prestwich, Jason A Burdick Abstract Hyaluronic acid (HA) is widely used in the design of engineered hydrogels, due to its biofunctionality, as well as numerous…
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July 29, 2020 in Publications

Application of Synthetic Polymeric Scaffolds in Breast Cancer 3D Tissue Cultures and Animal Tumor Models

Girdhari Rijal, Chandra Bathula, and Weimin Li Abstract Preparation of three-dimensional (3D) porous scaffolds from synthetic polymers is a challenge to most laboratories conducting biomedical research. Here, we present a handy and cost-effective…
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July 29, 2020 in Publications

A versatile 3D tissue matrix scaffold system for tumor modeling and drug screening

Girdhari Rijal and Weimin Li Abstract Most of the anticancer drug candidates entering preclinical trials fail to be approved for clinical applications. The following are among the main causes of these failures:…
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July 29, 2020 in Publications

Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture

Girdhari Rijal, Jing Wang, Ilhan Yu, David R. Gang, Roland K. Chen and Weimin Li Abstract Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of…
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July 29, 2020 in Publications

A multi‐site metastasis‐on‐a‐chip microphysiological system for assessing metastatic preference of cancer cells

Julio Aleman, Aleksander Skardal Metastatic disease remains one of the primary reasons for cancer‐related deaths, yet the majority of in vitro cancer models focus on the primary tumor sites. Here,…
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July 29, 2020 in Publications

Probing prodrug metabolism and reciprocal toxicity with an integrated and humanized multi-tissue organ-on-a-chip platform

Shiny Amala Priya Rajan, Julio Aleman, MeiMei Wan, Nima Pourhabibi Zarandi, Goodwell Nzou, Sean Murphy, Colin E. Bishop, Hooman Sadri-Ardekani, Tom Shupe, Anthony Atala, Adam R. Hall, Aleksander Skardal Current…
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July 29, 2020 in Publications

Model of Patient-Specific Immune-Enhanced Organoids for Immunotherapy Screening: Feasibility Study.

Konstantinos I. Votanopoulos MD, PhD, Steven Forsythe MSc, Hemamylammal Sivakumar MSc, Andrea Mazzocchi BSc, Julio Aleman BSc, Lance Miller PhD, Edward Levine MD, Pierre Triozzi MD & Aleksander Skardal PhD Introduction We hypothesized that engineering a combined lymph…
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